Paramecium Stain

ferry at mscd.edu ferry at mscd.edu
Wed Sep 15 13:07:35 EST 1999


"Donald O'Malley" <domalle at LYNX.DAC.NEU.EDU> wrote:

>     Does anyone know of a vital stain for paramecium?
>We're trying to image the movements of paramecium and
>want a stain that will provide either intense color or
>high contrast, so as to be easily visible in video frames.
>Longer term (several hours or longer) toxicity to the paramecium
>is not of any concern, but we would like them to be able to
>continue swimming for at least an hour or two,
>before going flagella-up.

>Donald M. O'Malley, Ph.D.
>Department of Biology
>414 Mugar Hall
>Northeastern University
>Boston, MA 02115
>domalle at lynx.neu.edu
>www.omalleylab.neu.edu


Well dilute solutions of Neutral Red, Genitian Violet or Methylene Blue
can be used.  Never used them so I can't tell you about the
concentrations to use  that  won't kill them.  Optical staining through
polarized light or Rheinberg illunination is available!


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