Paramecium Stain

ferry at ferry at
Wed Sep 15 13:07:35 EST 1999

"Donald O'Malley" <domalle at LYNX.DAC.NEU.EDU> wrote:

>     Does anyone know of a vital stain for paramecium?
>We're trying to image the movements of paramecium and
>want a stain that will provide either intense color or
>high contrast, so as to be easily visible in video frames.
>Longer term (several hours or longer) toxicity to the paramecium
>is not of any concern, but we would like them to be able to
>continue swimming for at least an hour or two,
>before going flagella-up.

>Donald M. O'Malley, Ph.D.
>Department of Biology
>414 Mugar Hall
>Northeastern University
>Boston, MA 02115
>domalle at

Well dilute solutions of Neutral Red, Genitian Violet or Methylene Blue
can be used.  Never used them so I can't tell you about the
concentrations to use  that  won't kill them.  Optical staining through
polarized light or Rheinberg illunination is available!

Sent via
Share what you know. Learn what you don't.

More information about the Protista mailing list