a stain for living cells?

Leonard Ornstein lenornst at pipeline.com
Tue Aug 22 07:26:59 EST 2000


Rosie:

Chilomonas is a very active flagelate which swims vigorously. It will
be very hard to grow them on agar if its too dry for them to swim. 
And
otherwise, they will not remain in cohesive colonies. 

You probably want a "vital" dye which doesn't kill them. I don't know
of a good one for your purposes. Of course there are numerous 
dyes for
staining killed and fixed Chilomonas.

Len Ornstein

Rosie Redfield wrote:
> 
> Hi,
> 
> I'm trying to grow Chilomonas (a cryptomonad) on agar plates.
> Unfortunately the cells are
> colourless, making any colonies that might arise very difficult to
> see.
> 
> Does anyone know of a dye that would bind to the cells without
> harming
> them?  I'd like to add such a dye to the agar to make small
> accumulations of cells more visible.
> 
> Thanks,
> 
> Rosie
> 
>   Dr. Rosemary J. Redfield      redfield at interchange.ubc.ca    | 
>   either email
>      rosie.redfield at zoo.ox.ac.uk      |  will work fine.
> 
>   On sabbatical until July 2001 at:
>            Dept. of  Zoology, University of Oxford, Oxford UK OX1
>            3PS lab phone (0)1865 281306; mobile phone (0)7730 368570
> 
>   Associate Professor               Department of Zoology
> 
>   Univ. of British Columbia       Office: (604) 822-3744
>   Vancouver, B.C. V6T 1Z4         Lab:    (604) 822-6323
>   Canada                          Fax:    (604) 822-2416
>   WWW site:  http://www.zoology.ubc.ca/~redfield
> 
> ---
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