[Pseudomonads] P. aeruginosa density

PiLS via pseudomo%40net.bio.net (by pils from invalid.ca)
Tue Sep 4 15:07:45 EST 2012


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Hi group,
We want to do some epithelium/bacteria interaction experiments.
(Pseudomonas aeruginosa, strains PA01 and PA14).

Our protocol for bacteria preparation involves a 3-hr
incubation of the bacteria in calcium-free PBS to prime type 3 
secretion before putting the bacterial cells on the epithelium.

We want to try different concentrations, and of course density
influences the pathogenicity profile.

The densities we are looking at are 10,000, 1,000 and 100
bacteria per microliter.

Would it be better to do the dilutions from the start, and
perform the incubations in PBS at the appropriate densities,
or to do a single incubation at high density and dilute that
according to the needs?

Thanks

- --
PiLS
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