SCF format and Staden's 1995 paper

Volodya BO-BA at mail.ru
Wed Mar 1 23:25:15 EST 2000


Hello,

	In the Staden's 1995 paper the SCF file format specification provides
all four (for A, C, G, and T) not just the highest, quality measures for
each basecall in a read. What happened with this requirement since 1995?
Since the present standard quality measure is the phred score, what is a
way to get four phred scores for each basecall? Phred documentation is
silent about this. Any pointers would be appreciated. 

Vladimir




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