Nigericin help

JAM jmarti at interport.net
Thu Jul 11 09:50:59 EST 1996


I have read conflicting information regarding the effects of nigericin
on live cells in normal physiologic media: (1) Dissipates _membrane
potential_ only, while not disrupting active transport or other
physiologic activities OR (2) Dissipates _pH gradient_ in addition to
potential and affects all cellular processes.  Again, this is under
normal conditions, not as a tool to make a standard curve for
intracellular pH measurements.  My ultimate goal is to dissipate or
equilibrate the pH gradient OR the membrane potential across the
plasma membrane and then measure uptake of a compound.  Any help would
be appreciated, 

James Martiney, Ph.D.
sherry.lab at picower.edu




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