antibacterial agent

Chuck Miller rellim at MAILHOST.TCS.TULANE.EDU
Tue Oct 21 10:56:39 EST 1997

>My brother-in-law has been recommended to use sodium azide (NaN3) as an
>antibacterial agent in his antibody-storage-medium.
>What concentration should he use?
>Azide is rather nasty, could he use anything else?

Sodium azide is usually used in buffers, etc., at a concentration of 0.1 to
0.2% to prevent microbial growth. People use azide because it does not
react with antibodies, nucleic acids, and other biological probes. Other
anti-microbial agents may contain detergents or other compounds that are
detrimental to experiments--so choose alternatives carefully.

Best of results,


Dr. Charles A. Miller, rellim at
Bionet.toxicology news group,
Dept. Environmental Health Sciences, SL29
Center for Bioenvironmental Research
Tulane Univ. School of Public Health and Tropical Medicine
1430 Tulane Ave.
New Orleans, LA 70112

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