Axolotls out of water

Natalie Kim Bjorklund umbjork1 at cc.UManitoba.CA
Thu Apr 27 11:02:26 EST 1995


We want to be able to use MRI to image the axolotls during normal limb 
regeneration. This will require having the limb in question exposed to 
the air for extened periods perhaps as much as an hour. I was wondering 
if anyone has any experience with this?
We are considering wrapping the animals in wet clothes and placing them in 
a high humidity enviroment in a closed chamber for imaging with the limb 
of interest exposed. We are wondering if this will be sufficient for the 
animals. We are also considering a water filled chamber with the leg 
sticking up in the air but this is more likely to damage the MRI coil by 
water exposure.
Has anyone done any experimentation to see how long an anesthetized 
animal can survive out of water, in shallow water etc. We have had an 
axolotl jump out of it's tank and survive about a half an hour out of the 
tank without any apparent ill effects in an accident. But our animals are 
precious and we don't want to risk losing any of them. Any suggestions 
would be very much appreciated.

Natalie K Bjorklund



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