Endogenous AP's

Chris D'Jamoos djamooca at flyernet.udayton.edu
Fri Aug 23 13:14:19 EST 1996


I was wondering if anybody has tried (Genius 4) DIG in situ 
hybridizations using the Alkaline phosphatase anti-congugate with frozen 
sections.  Apparently, these sections are levamisole resistant and 
methods such as Acid Alcohol, 5mM EDTA/DEPC at 65 degrees, and 
increasing Lev. concentration does not decrease this masking background. 
 I am working with Xenopus tissue from the tadpole stages through 
metamorphosis and have not found any literature for doing  nonradiactive 
in situ hybridizations on xenopus frozen sections.  If anyone has any 
ideas or leads please pass them on.  I am currently looking at other 
anticonjugates, as well as, my last resort- radioactive methods.

Thanks in advance,

Chris D'Jamoos




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