demembranation of sperm

Matthew D. McLean MCLEANM at VAX.CS.HSCSYR.EDU
Wed Apr 9 17:00:48 EST 1997


Has anyone tried demembranating sperm from the Mexican Axolotl?

I have been trying lysolecithin but as of yet to no avail.

I am currently in the process of trying a more dilute preparation of sperm
under the assumption that the extensive tail and its associated membrane
might be hinering the demembranation by taking the lysolecithin out of 
action.

Anyone information regarding this would be greatly appreciated.

Sincerly,
Matt McLean

SUNY HSC @ Syracuse
750 East Adams St.
Syracuse, NY  13210
(315)-464-8539
MCLEANM at VAX.CS.HSCSYR.EDU




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