[Urodeles] Anesthetics and Analgesics for Axolotls

Stanley Sessions via urodeles%40net.bio.net (by sessionss from hartwick.edu)
Fri Apr 2 11:55:40 EST 2010


Whoops!  Make that 0.1% MS222 (dissolved in 0.2% sodium bicarb.).

Pura Vida,

Stan
Stanley Sessions PhD
Department of Biology
Hartwick College
Oneonta, NY 13820-1904
607-431-4764
sessionss from hartwick.edu







On Apr 2, 2010, at 11:09 AM, Stanley Sessions wrote:

> Axolotls (and other salamanders) do not appear to need a post- 
> amputation analgesic (other than, perhaps, keeping them cold for a  
> while). My assay is feeding:  they usually commence feeding, as if  
> nothing happened, as soon as they recover from the anesthetic.  I  
> don't think that would happen if they were in severe post-amputation  
> pain.
>
> I have found nothing that comes close to MS222 for safely  
> anesthetizing axolotls or any salamander or fish (we use 0.3% for  
> newts). It is reasonably rapid (takes 15-20 minutes for adult  
> newts), gentle yet profound and long-lasting, with a steep recovery  
> curve. However, plain MS222 dissolved in water has a very low pH  
> which can cause significant discomfort to the animal (judging by the  
> fact that they thrash around). So I recommend buffering it (e.g.  
> with 0.2% sodium bicarbonate). Another advantage of (buffered) MS222  
> is that you can make a large quantity of it and keep it in the  
> refrigerator. Warm to ambient temperature (to match the water that  
> the animal is in) before using, however.
>
> Pura Vida,
>
> Stan
> Stanley Sessions PhD
> Department of Biology
> Hartwick College
> Oneonta, NY 13820-1904
> 607-431-4764
> sessionss from hartwick.edu
>
>
>
>
>
>
>
> On Apr 18, 2009, at 4:07 AM, Jordi Cantó wrote:
>
>> Hello Natalie,
>>
>> Please, can I have the summary of responses to these questions:
>>
>> 1) Do axolotls need a post leg amputation analgesic? If not why  
>> not? If they do, what sort is appropriate? For how long into the  
>> regeneration period do they require painkillers?
>>
>> 2) Is there another anesthetic other than MS222 appropriate for  
>> anesthetising the axolotls for such an amputation?
>>
>> Thanks,
>>
>> -- 
>> Jordi Cantó Martorell
>> Director Servei d'Estabulari - SIAL
>> Universitat Autònoma de Barcelona
>> SPAIN
>>
>>
>> _______________________________________________
>> Urodeles mailing list
>> Urodeles from net.bio.net
>> http://www.bio.net/biomail/listinfo/urodeles
>
>
> _______________________________________________
> Urodeles mailing list
> Urodeles from net.bio.net
> http://www.bio.net/biomail/listinfo/urodeles




More information about the Urodeles mailing list