ajeffrie at waite.adelaide.edu.au
Thu Dec 10 04:12:00 EST 1992
I think it is great that this group is up and running but it seems to be
a bit slow. Well instead of complaining I'll do something....
Fact1: Virus "species" are best described as a population of minor
sequence varients. The term quasispecies is usually used here.
Fact2: PCR amplification has an error rate associated with it. The rate
is dependent on the type of polymerase used and other factors such as Mg-2+
concentration and the number of cycles.
Question1: When sequencing a virus, where the clones have been
generated by PCR, how many instances of a particular sequence would have to be
seen before people here would be happy that they have the "correct" sequence and
not a PCR artifact.
Thanks in advance,
Prof. R. H. Symons' Lab.
Dept. Plant Science ajeffrie at waite.adelaide.edu.au
Waite Agricultural Research Institute
Glen Osmond, South Australia
(The University of Adelaide)
More information about the Virology