Iridoviruses

Dan Jacobson danj at welchgate.welch.jhu.edu
Tue Mar 2 12:25:00 EST 1993


In article <ashok.458.0 at biochemistry.cwru.edu> ashok at biochemistry.cwru.edu (Ashok Aiyar) writes:
>In article <CHRIS.93Mar1172826 at scan.scan.genetik.uni-koeln.de> chris at scan.genetik.uni-koeln.de (Christian Schetter) writes:
>
>>Anybody around working with iridoviruses?
>
>I know someone at Cornell -- I think his name is James Casey, and he works 
>with piscine (sp ?) -- anyway fish :-) retroviruses and iridoviruses.
>
>>Is even someone reading this newsgroup?
>
>Yes.
>
>Ashok
>
>

Here is an abstract of what Casey's lab is doing.  You can search 
through all the abstracts of everybody being funded by NIH via gopher
at merlot.welch.jhu.edu in the 

      14. Searching For Biologists/

directory.

If you've never heard of gopher write me a note and I'll send you some
information to get you started (don't worry gopher is free and on the net :-).

Happy searching,

Dan Jacobson

danj at welchgate.welch.jhu.edu


-----------------------------------------------------------------------------


-PROJECT NUMBER......1 R01 CA53623-01A3

PRINCIPAL INVESTIGATOR

CASEY, JAMES W
CORNELL UNIVERSITY
123 DAY HALL
ITHACA, NY  14853
PERFORMING ORGANIZATION: CORNELL UNIVERSITY ITHACA
TITLE   Retroviral etiology of walleye dermal sarcoma

ABSTRACT:

 The goal of this proposal, is to obtain fundamental information with
 regard to the structural and functional organization of the walleye
 dermal sarcoma virus (WDSV). We will utilize these data to examine the
 epidemiological and molecular mechanisms responsible for the development
 and regression of tumors in both experimental and natural infections of
 walleyes.  Using defined, molecular and immunological probes, we will
 follow the fate of WDSV DNA, RNA and viral proteins in these tumors.  In
 addition to providing information as to the prevalence of infection in
 natural fish populations, data from these analyses should provide further
 insights into the molecular mechanisms responsible for tumorigenesis.  We
 propose to determine the complete nucleotide sequence of a full-length
 molecular clone of WDSV.  This will be accomplished using the Sanger
 dideoxy sequencing procedure and will provide a foundation data base for
 subsequent identification of transcriptional processing signals, and the
 presence of nonstructural viral accessory genes.  Northern blot analysis
 and the construction and sequencing of viral cDNAs will be used to
 establish a precise transcriptional map of the WDSV genome.  We place
 heavy emphasis on this approach in that our current data support the
 hypothesis that a viral-encoded function, analogous to TAX and TAT of
 HTLV-1 and HIV, respectively, is responsible for tumor induction in
 walleyes.  Concurrent with these studies, we propose to investigate the
 in vitro tropism of WDSV through infection studies and DNA transfection
 experiments.  A major aim will be to establish a cell culture system for
 WDSV and use this system to investigate transcriptional regulation and to
 characterize biologically active molecular clones of WDSV.  To carry out
 these studies we plan to develop specific immunological reagents to
 follow the fate of virus, both in vivo and in vitro.  An extension of the
 current in vivo transmission studies will identify the in vivo cellular
 targets of WDSV, routes of infection and eventually allow for induction
 of tumors by well-characterized viral molecular clones.



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