spontaneous mutation rate in RNA viruses

Ed Rybicki ED at micro.uct.ac.za
Thu Mar 4 04:59:04 EST 1993


> Hi Vironetters,
>
> I am in need of two protocols. The first one utilises CF-11 (Whatman) to
> isolate ds RNA, the second one uses differential salt precipitations (I
> think 2M and 4M, as well as ethanol). Finally, does anyone have any
> information on the use of glycogen in dsRNA precipitations ?

So - life in virology at net.bio.net!

The best dsRNA extraction protocol - one which works for big plant virus
RNA genomes, as well as 9kb insect picornavirus genomes (Williamson et al.
1988, J gen Virol 69:787-795) - is that of Valverde et al. 1986,
Phytopathology 76:459-465.  The refs. describe use of Whatman CF11
cellulose columns/batch procedures for isoln of dsRNA replicative
forms/intermediates from plants and insects.

Good luck!  And greetings from the other Down Under.

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