Green Fluorescent protein

Robert Means remeans at husc9.harvard.edu
Fri Jun 3 08:04:06 EST 1994


Hello,
	I'm currently constructing an retrovirus vector for expressing 
GFP and this thread disturbs me. Does anyone know for sure whether the 
problem is at the expression level or the detection level? In preliminary 
experiments, we had problems observing expression in transient 
transfections, but we thought that was just a problem of low transfection 
efficiency. I guess I'll just watch this thread for answers.


							Thanks,
								Bob Means

New England Regional Primate Research Center...Where the men are men and 
the monkeys are nervous.




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