fd phage expression

Andrew, Tel. +39-6-91093434 WALLACE at IRBM.IT
Thu Mar 17 02:30:37 EST 1994


Message forwarded from Franco Felici {felici at irbm.it}

>From:	IRBMV1::FELICI       16-MAR-1994 12:48:39.09
>To:	WALLACE
>CC:	
>Subj:	se puo' essere di interesse generale...
>
>    #59         14-MAR-1994 13:05:13.77                              PEPLIBS
>From:   IRBMV1::FELICI
>To:     SMTP%"plyhmb at pln1.nott.ac.uk"
>CC:     FELICI
>Subj:   RE:fd phage expression

>Dear Mark,
>
>from my point of view, it can not be predicted "how large a polypeptide can be
>expressed, fused to the N terminal of the gene III product, without
>significantly affecting its function".
>As you know, the N-terminus of pIII is important for phage infectivity, I
>think that the size of the N-terminal fusion is not the only thing that has
>to be considered, also its expression, folding, capability to be translocated,
>composition, stability in E. coli are very (if not more...) important.
>A "strange" peptide could be worse than a large protein for pIII function.
>
>Best wishes for your work.
>
>                                        Franco Felici



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