"LONG" RT/PCR

JEAN at BIO.TAMU.EDU JEAN at BIO.TAMU.EDU
Wed Nov 16 11:10:15 EST 1994


To:            virology at net.bio.net
          
Subject:       Re: "LONG" RT/PCR
Date:          16 Nov 1994 15:05:21 GMT




I have also found that the RT reaction is limiting.  Typically I do RT 
extensions for 1 1/2 hrs.  I have also found that the primers can be 
limiting so adding additional excess 3' primers to the RT or PCR reactions 
can increase the amount of products 

JV



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