"LONG" RT/PCR

Hassan BADRANE hbadrane at pasteur.fr
Wed Nov 16 10:05:21 EST 1994


hello 
We have tryed what Elizabeth Estabrook suggested to you and we got the best
results with the Superscript (Gibco BRL) and by using DTT (because we have
tested many Enzymes and with or without using DTT and RNASIN).

I can also advice you to try the Taq polymerase made by Amersham "tube", it's 
characterized by doing a long amplifications.

GOOD luck



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