PCR-Ligation

Dr Michael J Carter bss1mc at surrey.ac.uk
Mon Nov 21 04:37:08 EST 1994


Hi y'all,

ecently there has been a lot of discussion re long PCRs.
Well extending this a bit, has anyone any good tips
on PCR ligation? IE joining two overlapping DNAs by PCR
using primers at the extreme ends of each molecule?

Specifically, how many (if any) cycles do you do before
adding the primers (ie to build the full-length before you 
amplify).
Do you need to change the conditions much?

All help greatly received, currently I am missing
the magic ingredient (15mM oofle-dust??)

Cheers
Mike
:-)




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