Intravirion nucleic acid replication

Jarmo Juuti juuti at cc.Helsinki.FI
Wed Aug 30 14:20:57 EST 1995


Karl asks:
> My question is - What other viruses demonstrate intravirion replication
> of their genome as opposed to ex virion synthesis then encapsidation?

Dear Karl,

	I may be out of your interest area but, I just comment that the
dsRNA bacteriophage phi6 (which I play around with) packs genomic plus-strand
RNA segments (three;S,M,L) into preformed procapsids. After packaging
minus-strands are synthesized inside the procapsids leading to dsRNA.
After that the system can go to plus-strand synthesis, i.e.
transcription, directly and that's again inside the particle.

	The interesting point here for the "eucaryotic" people is that
viruses most resembling phi6 are reoviridae members (reo-, rota- and
orbiviruses). With them it has not been shown yet that the case would be
the same, but it sure looks like it (at least to me). They are closest
to show it with rotaviruses as they now have the improved in vitro
replication system (J. Virol. 68:7030-7039).

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