PCR contamination

James Scutero jscutero at panix.com
Tue Feb 21 10:57:39 EST 1995

T.J. Fitzmaurice writes:
>>Have you done a comparision of the
>>genetic sequences of KSHV and HHV-6(A and B) using GENEBANK?
>Yes I have actually. Here are the results. There are two published sequnces
>in KSHV, one larger than the other. The second smaller fragment carried
>similarities to a few minor human transferase genes. the larger one was more
>Homology is listed to several genes in Epstein Barr Virus, running at about65%
>homology. Next in order of similarity came Herpvesvirus saimiri,Bovine herpes4
>AHV-1, HSV-1, human cytomegalovirus and then Equine herpes virus 1.
>HHV-6 is nowhere on that list..... Sorry
>Go check it yourself.
>>I THINK you should tell me how these two viruses, HHV-6 and KSHV, 
>>differ physically and genetically.
>>-James M. Scutero
>Well since you seem to be putting them together, the onus unfortunately
>is on you to prove their indenticality on these issues. Similar they may be
>but the evidence they are not the same.See the genbank results I quote above.
Thank you for taking the time to answer my questions. KSHV is not a variant
of HHV-6.

-James M. Scutero

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