chale at hsc.usc.edu
Fri Jan 27 13:32:17 EST 1995
zxy5 at po.CWRU.Edu (Zafer Yildirim) writes:
>We are using recombinant retroviruses for gene transfer to some
>mammalian cells. We include polybrene during the infection of
>these cells with the viruses. Can somebody tell
>me how polybrene works to enhance infection?
>Thanks in advance.
The theory that I have heard is that polybrene coats membranes and thus shields
the negative charges on the surface. This allows membranes to come together
more easily and facilitates viral fusion. Unfortunately I don't have any referances
that support this, but you do see enhanced cell-cell fusion in the presence of
polybrene which gives some support to this idea. Hope this helps a little.
USC Department of Biochem.
"No Pain, No Pain!!!"
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