Does HIV Exist (fowarded from sci med aids) 3/3
weissm at rockvax.rockefeller.edu
weissm at rockvax.rockefeller.edu
Thu Mar 9 16:34:49 EST 1995
Date: Thu, 9 Mar 1995 14:05:46 -0600
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From: sma at wubios.wustl.edu
Subject: Does HIV exist?
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diseases. Even if the virus could have been isolated from all patients,
given the nature of retroviruses and the method used for HTLV-III isolation
(cultures, mitogenic stimulation, co- cultivation) the possibility cannot be
excluded that the virus did not exist in vivo (in AIDS patients), and that
it was a provirus whose expression was facilitated by the culture
conditions. The only method used to prove HIV infection in vivo was the
antibody tests. Such a test can only be used only after its specificity has
been proven by use of the only possible gold standard, the virus itself.
This has not been done.
Furthermore, the antibody test used by Gallo was ELISA, at present known to
be non-reproducible and non-specific. In a study of 1.2 million healthy
military applicants conducted by Colonel Donald Burke and his colleagues,63
it was found that although approximately 1% of all individuals had an
initial positive HIV ELISA, only 50% of repeat ELISAs were positive. Of the
latter, only approximately one third were associated with two subsequent
positive WBs. In Russia, in 1990, out of 20,000 positive ELISAs "only 112
were confirmed" using the WB as a gold standard. In 1991, of approximately
30,000 positive ELISAs, only 66 were confirmed.64
Nowhere in the four Science papers was HTVL-III cytotoxicity mentioned. The
only reference to any cellular abnormalities or pathology in general is in
the first paper where one reads: "The virus positive cultures consistently
showed a high proportion of round giant cells containing numerous nuclei
(Fig. 1a). These cells resemble those induced by HTLV-I and -II except that
the nuclei exhibit a characteristic ring formation". (Fig. 1a is a "light
microscopic examination of clone H4/HTLV-III"). The H4 clone was obtained
from the HT cell line "using irradiated mononuclear cells from peripheral
blood of a healthy blood donor as a feeder". At present, it is known that
the HT cell line and thus H4 are HUT78, derived in 1980 from a patient with
mature T4-cell leukaemia,65,66 However, other cell lines derived from
patients with the same clinical syndrome are known to exhibit similar
morphologies including multinucleated giant cells.67 Thus the cellular
morphological characteristics observed in the first paper may have been an
intrinsic property of the HT cell line, or the result of the culture
conditions, or both, and not due to HTLV-III.
Finally, Gallo and his colleagues did not provide any data on the
immunological status of those individuals from whom viral isolation was
attempted, and no data was presented proving that:
1. HTLV-III (HIV) is both a necessary and sufficient cause of T4- cell
2. T4-cell depletion is both necessary and sufficient for the appearance of
the AIDS indicator diseases.
CONCLUSIONS. The data and arguments that have been presented by Gallo and
his colleagues do not constitute proof of HIV isolation or an unambiguous
role for HIV in the pathogenesis of AIDS. Although some researchers
currently use methods of "viral isolation" essentially the same as that
described by Gallo's group, most use less rigorous methods including
singleton detection of p24 (by antibody techniques), or RT. Notwithstanding,
with all of these techniques, including that described by Gallo and his
colleagues, which itself seen to be greatly problematic, HIV cannot be
"isolated" from 20%-70% of HIV positive and AIDS patients.68,69 Thus we are
faced with a problem of considerable importance. The HIV antibody tests,
both ELISA and WB, the only routinely used tests proving the existence in
vivo of HIV, have yet to be verified against the only suitable gold
standard, viral isolation. The available evidence suggests that this long
overdue but most basic requirement of test evaluation is likely to prove an
immense problem, and while the HIV antibody tests are useful prognostic
markers in the high risk groups, their use as diagnostic and epidemiological
tools for HIV infection is questionable.
Footnote 1. In the Western Blot test, proteins are electrophoretically
separated according to molecular weight and charge. The separated proteins
are then transferred on to nitrocellulose strips by a process known as
electroblotting. When sera are added and the strips developed, coloured
bands appear representing sites of protein/antibody reactions. Each band is
designated by a small "p" for the protein followed by its molecular weight
Footnote 2. In the ELISA (Enzyme Linked Immunosorbent Assay), unseparated
proteins are attached to a solid base such as the walls of plastic tubes or
microplates. The serum being tested is incubated in these containers where
antibody is fixed to the solid phase antigens. After washing,
enzyme-labelled anti-human immunoglobulin is added and also incubated. The
containers are again washed and a substrate specific for the enzyme is
introduced. The resulting colour change is proportional to the amount of
antibody present and is read by eye, or with a spectrophotometer.
ACKNOWLEDGEMENTS We wish to thank all our colleagues and especially Udo
Sch=81klenk, Barry Page, Bruce Hedland-Thomas, David Causer, Richard Fox,
John Peacock, David Prentice, Ronald Hirsch, Patricia Shalala, Keith Jones,
Alun Dufty, June Rider Jones, Coronary Barrow, Dorothy Davis, Julian Smith,
Mark Strahan, Vincent Turner, Wallace Turner, Gary James and Graham Drabble
for their continued support and assistance.
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