Regarding the infectivity of naked DNA
Ian A. York
york at mbcrr.dfci.harvard.edu
Tue May 23 16:23:31 EST 1995
In article <wardp-2305951340410001 at oisin.usask.ca> wardp at herald.usask.ca (Pearse Ward) writes:
>The implication from this work is that the immune response generated is
>cell-mediated immunity. Without antigen recognition by CD4+ cells, I don't
>see how this is possible. Therefore, for this system to be working as
>advertised, there must be some explaination as to how the exogenous
>antigen gets presented to Th cells.
CTL can, in some cases, be primed with no contribution from CD4 cells;
see the references below. CTL priming is a mysterious process, and I
believe that sometimes helper cells are clearly required, sometimes they
are not. That leaves the naked DNA priming still open. By the way, I
believe Ulmer's paper specifically identified CTL specific for the gene
Rahemtulla A. Fung-Leung WP. Schilham MW. Kundig TM. Sambhara
SR. Narendran A. Arabian A. Wakeham A. Paige CJ. Zinkernagel RM. et al
Normal development and function of CD8+ cells but markedly
decreased helper cell activity in mice lacking CD4.
Nature. 353(6340):180-4, 1991
"Here we report that these mice have markedly decreased helper cell
activity for antibody responses, although cytotoxic T-cell activity
against viruses is in the normal range."
Rahemtulla A. Shahinian A. Kundig T. Zinkernagel R. Mak TW.
CD4 negative mice as a model for immunodeficiency.
Philos Trans R Soc Lond B Biol Sci. 342(1299):57-8, 1993
"A mouse strain without CD4 expression has been generated. These animals,
surprisingly have a normal CTL response and reduced, but not absent,
Ian York (york at mbcrr.harvard.edu)
Dana-Farber Cancer Institute, 44 Binney St., Boston MA 02115
Phone (617)-632-3921 Fax (617)-632-2627
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