Westerns on uninfected H9 cells

Mr. Goodbar glenalan at mars.superlink.net
Thu Oct 26 12:53:09 EST 1995


brett wrote:

> glen, since when are negative controls useless?!?
> 
> Brett Lindenbach

	Negative controls are not useless,  the kit comes with a 
strong ab+, weak ab+ and an ab-.  Due to performance of the 
current assay, you won't see any H9 reactivity.  It would be like 
running an uncoated well in an ELISA.  There may be non-specific 
binding in the well, but the postcoating process would eliminate 
that.  Therefore, no need.



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pub   768/CA963CBD 1995/03/25 Glen A. Pannicke 
<glenalan at mars.superlink.net>
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B7



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