Help!PCR primers for SV40 large T antigen?

Robert Hnasko hnaskor at ucunix.san.us.edu
Fri Sep 8 14:59:44 EST 1995


I am looking for sucessful PCR primers for the SV40 large T antigen.  If 
anyone has a reference or a protocol please email ASAP.  I have been 
unsuccessful in my literature search for anyone using primers for the 
large T.  I would like to genotype heterozygote transgenics in this 
manner.  We are trying to limit the need to run southerns do to time, 
money, and management.  Thanks 

hnaskor at ucunix.san.uc.edu

Robert Hnasko
University of Cincinnati





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