Collagen beads and tissue culture

Bart_Corsaro_at_USLRMG01 at INTERNETMAIL.PR.CYANAMID.COM Bart_Corsaro_at_USLRMG01 at INTERNETMAIL.PR.CYANAMID.COM
Wed Sep 20 07:07:34 EST 1995


Vince,

Have you thought about using VERO cells attached to beads in a spinner culture.  
You can get a higher cell density/ml of culture.

Bart Corsaro
Wyeth-Lederle Vaccines and Pediatrics
211 Bailey Rd.
West Henrietta, NY, USA 14586-9728
Email: Bart_Corsaro_at_USLRMG01 at internetmail.pr.cyanamid.com

_______________________________________________________________________________

>I have a question to any who is concerned.  Actually, I would 
>like to increase the number of vero cells in tissue culture flasks (75 
>cm^2) using collagen beads, but have never done so.... Has anyone used 
>the collagen beads to increase cell concentrations?  If so, how much 
>greater is the yield?  Yes, I could probably order larger flasks but must 
>use what I have.  The purpose is to increase yields of enteric viruses 
>which max at 10^5 viruses/cell.  Any and all insight is welcome.  Thanks.
>
>Sincerely, 
>
>Vincent J. Magrini





More information about the Virology mailing list