Hanks vs PBS

Keith Appleyard K.APPLEYARD at dundee.ac.uk
Mon Jul 29 08:46:36 EST 1996


As a buffer solution ALONE (i.e not as part of other formulations) PBS
is used as a generally applicable buffer (washing cell monolayers,
viral dilutions etc) whilst the more complex Hanks buffer is reserved
for more critical uses (such as tissue disaggregation etc).

The Basic question is why ???

I am fully aware of both formulations, PBS simple + no carbon source ;
Hanks more complex + carbon source (glucose) but what is the reasoning
behind their differing use, wouldn't just one or the other be
generally better overall? As usual the documented answer seems lost in
history with current generations of virologists simply accepting
stated use without question. Anyone out there know better??

Any answers much appreciated to this.
Thanks in advance.
Keith Appleyard, Senior Scientific/Dept.Safety Officer, 
Virology, Ninewells Hospital and Medical School, Dundee DD1 9SY,U.K
e-mail k.appleyard at dundee.ac.uk Tel:U.K 01382 632559 Fax:01382 641907



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