HELP: Why 50bp marker is a smear in denatured gel?

Bixing Huang huangbx at DEAKIN.EDU.AU
Thu Jun 20 20:45:16 EST 1996


Dear netters,

I am trying to get denatured gel  (5M urea) running in my lab. The problem I
am encountered is the 50bp DNA marker is a smear after silver stained.

If you can share your successful protocol or have any suggestions, I would
be very grateful to you.

Thank you very much in advance.

Bixing Huang

Department of Biology, Deakin University of Australia.
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