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Purification of HSV virions

Christian Schetter chris at scan.genetik.uni-koeln.de
Mon Oct 14 11:13:16 EST 1996


We are looking for a reliable method to purify HSV-1 virions. Although
we tried density centrifugation using Ficoll-400 we could not obtain
banded virions. The suspicion is that the paramters from the
literature or the calculation for our Beckman centrifuge are not
correct. We would greatly appreciate any hint etc.

Chris

Christian Schetter
Max-Planck-institut fuer Biochemie
Martinsried
e-mail: schetter at biochem.mpg.de





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