Miss Golden Clone
zxm at pasteur.fr
Thu Sep 12 11:15:28 EST 1996
today I have a really serious question t everyone beeing familiar with
Here my problem:
Since more than a century now, I am trying to clone a PCR fragment, which
I designed XhoI-XhoI, into an Xho-NheI. However, since today I have not
been able to get a single positive clone. Why?
Does anyone has valuable suggestions, I really would appreciate it.
Thanks in advance.
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