Cloning problems

Miss Golden Clone zxm at pasteur.fr
Thu Sep 12 11:15:28 EST 1996


Hi everybody,

today I have  a really serious question t everyone beeing familiar with
molecular biology.
Here my problem:

Since more than a century now, I am trying to clone a PCR fragment, which
I designed XhoI-XhoI, into an Xho-NheI. However, since today I have not
been able to get a single positive clone. Why?
Does anyone has valuable suggestions, I really would appreciate it.
Thanks in advance.





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