pTracer stability without large T antigen???

Guy Hermans ghermans at luc.ac.be
Wed Apr 16 11:37:58 EST 1997


I'm considering of using the new pTRACER vectors of Invitrogen. It are
vectors with an SV40 ori and as tracer GFP. I'm planning of using them to
express a gene in lymphocytes. 

My questions:

1. Is it possible to use this vector in transfections of T lymphocytes?
They don't have the large T antigen, and I wondered if the T antigen is a
necessary factor? It doesn't say anything in the folder I got. What will
theoretically happen with the SV40 plasmid vector? Will it replicate
together with the cells?  It is possible to select for an antibiotic.

2. Does anyone have experience with this at all?


Thanks for the help
niels hellings
-- 
  Niels Hellings, PhD student
  Ms research Unit              Immunology research group
  Dr. L. Willems-Institute      Dept. of Physiology, LUC
  University Campus             University Campus
  B-3590 Diepenbeek             B-3590 Diepenbeek
  Belgium                       Belgium
Voice  ++32(0)11/26.92.07
Fax    ++32(0)11/26.92.09

Niels Hellings




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