Hopkins Grad Seeks Post-Doc in HIV/AIDS Research

Michael A. Egan Michael.Egan at jhu.edu
Tue Jan 7 03:47:37 EST 1997


                                Department of Molecular Biology and
                                Genetics Johns Hopkins University
                                School of Medicine Ross Research
                                Bldg., Rm. 1050 720 N. Rutland Ave.
                                Baltimore, MD 21205



January 5th, 1997

To whom it may concern:

I am writing in regards to potential post-doctoral / entry level
positions that may be available in the areas of basic science AIDS
research and HIV vaccine development.

I am a 5th year Ph.D. candidate in the Biochemistry, Cellular and
Molecular Biology Training Program at the Johns Hopkins University
School of Medicine.  I plan to complete my graduate program in the
spring/summer of 1997.

My research, under the direction of Robert F. Siliciano M.D. Ph.D.,
has been focused in two areas related to HIV/AIDS research.  I
developed a novel method (AIDS Research and Human Retroviruses, 10:
1423, 1994) for the detection and evaluation of cytolytic T-cell
responses to candidate AIDS vaccines being tested in Phase I clinical
trials at the Center for Immunization Research here at Johns Hopkins
(J. Infect. Dis. 171: 1623, 1995).  This method is now widely used in
the testing of AIDS vaccines.  

More recently my thesis work has focused on developing a better
understanding of the role of a highly conserved YXXL internalization
motif within the envelope glycoprotein gp41 of HIV-1 in viral
assembly, host immune recognition, and CD4 cell loss (J. Virol.,
10:6547-6556 (1996)). 

I seek a post doctoral / entry level position in the area of basic
sciences AIDS research and HIV vaccine development. My nine years
research experience, my excellent writing and verbal skills, along
with my proven ability to publish in peer reviewed journals makes me
an excellent candidate.   

I welcome an opportunity to discuss my capabilities with respect to
opportunities that may exist in this area.  I have enclosed a copy of
my curriculum vitae along with the name and addresses of three
references for your information.  

Please feel free to contact me by mail at the above address or by
telephone or E-mail:

        Phone: 410-955-7757
        Fax: 410-955-0964
        email: egan at welchlink.welch.jhu.edu
   http://www.welch.jhu.edu/~egan

Sincerely,


Michael A. Egan
----------------------------------------------------------------------
----

                                CURRICULUM VITAE


Name:                           Michael A. Egan

Current Appointment:            Ph.D. Candidate, Graduate Program in  
 
                                Biochemistry, Cellular and Molecular
                                Biology,   Johns Hopkins University,
                                School of
Medicine,                                           
                                Baltimore, MD

Office Address:                 Johns Hopkins University School of
Medicine,
                                Department of Medicine
                                Rm. 1050 Ross Research Bldg.
                                720 N. Rutland Ave.
                                Baltimore, MD 21205
                                Phone: 410-955-7757
                                Fax: 410-955-0964
                                e-mail: egan at welchlink.welch.jhu.edu
                                http://www.welch.jhu.edu/~egan

Home Address:                   3721 Rexmere Rd.
                                Baltimore, MD 21218
                                Phone 410-366-3268

EDUCATION

1992-present                    Ph.D. Candidate, Graduate Program in  
 
                                Biochemistry, Cellular and Molecular
                                Biology    (BCMB), Johns Hopkins
                                University, School of     Medicine,
                                Baltimore, MD

1987-1992                       B.S., Chemistry, Magna Cum Laude, 
                                Northern Arizona University,
                                Flagstaff, AZ


                            HONORS AND AWARDS

Undergraduate                   Magna Cum Laude in Chemistry
Education                       Grade Point Average 3.80/4.00
                                NSF Summer Research Fellowship
                                Golden Key National Honor Society Phi
                                Lambda Upsilon Chemistry Honor Society
                                Phi Kappa Phi Honor Society Dean's
                                List seven semesters

Post Graduate                   Fellowship, Graduate Program in
Biochemistry,   Education                       Cellular and Molecular
Biology (BCMB), Johns 
                                Hopkins University School of Medicine



                                WORK EXPERIENCE

1988-1991                       Undergraduate Chemistry lab teaching
assistant,
                                Northern Arizona University

Summer 1991                     NSF Research Fellowship, University of
Georgia

1983-1987                       Enlistment in the U.S. Marine Corps,
honorable 
                                discharge.


                           RESEARCH EXPERIENCE

1992-Present                    Elucidating the role of the YXXL
internalization 
                                motif within the envelope protein
                                (gp41) of HIV-1 in viral assembly,
                                immune
recognition, and 
                                CD4 cell loss.  Developed a novel
                                method,
now                                   
                                widely used in the AIDS field, for the
detection        
                                of (HIV-1)-specific CTL responses in
                                human vaccine recipients. In a Phase I
clinical                                        
                                trial evaluated the ability of an
                                experimental HIV-1 vaccine to elicit
                                HIV-specific CTL responses in
                                sero-negative volunteers.

                                Laboratory of Robert F. Siliciano,
                                Department of Medicine, Johns Hopkins
                                University School of Medicine.

1991-1992                       Using semi-empirical computational
methods to 
                                calculate the structure and electronic
                                properties of the mono-boro-analogs of
                                acetylcholine. 

                                Laboratory of Dr. Robert W. Zoellner,
                                Department of  Chemistry, Northern
                                Arizona University

NSF 1991 Summer                 Mutagenesis of murine ferrochelatase
in looking Fellowship                      for molecular mimicry in
erythropoietic 
                                protoporphyria.

                                Laboratory of Dr. Harry A. Dailey,
                                Department of Microbiology, University
                                of Georgia

1988-1992                       The effect of endurance training on
glucose 
                                metabolism.

                                Laboratory of Dr. Scott Savage and Dr.
Hans M.          
                                Gunderson, Exercise Physiology Lab,
                                Northern Arizona University



SKILLS

Trained in standard molecular biology techniques, PCR, SDS-PAGE,
Western Blot, Pulse Chase Analysis, the use of Fluorescence Activated
Cell Sorter analysis (FACS), all aspects of human tissue culture, and
optimization and implementation of standard immunological assays (Cr51
release, CTL assays, ADCC, ELISA). Five years experience in the
handling and use of high titer infectious material (HIV-1, vaccinia
virus, EBV) under Biohazard Level 3 (P3) laboratory conditions.
Computer trained with experience programming in FORTRAN, C/C++, HTML.
Skilled in the use of DOS/Windows, Mac and Unix operating systems.
Able to efficiently access and publish information on the WWW and
Internet. 


PUBLICATIONS

1. Michael A. Egan and Robert W. Zoellner.  Structural and Electronic
Characteristics of the Monoboro-Analogs of the Acetylcholine Cation As
Determined by the Semiempirical MNDO Computational Method.  J. Org.
Chem., 58:1719-1729 (1993).

2. M.N. Lubaki, Michael. A. Egan, Robert. F. Siliciano, Kent. J.
Wienhold, and Robert. C. Bollinger. A Novel Method for the Detection
and Expansion of HIV Type 1-Specific Cytolytic T Lymphocytes. AIDS
Res. Hum. Retroviruses, 10:1423-1427 (1994).

3. Michael A. Egan, Wendy A. Pavlat, James Tartaglia, Enzo Paoletti,
K. J. Wienhold, Mary Lou Clements, and Robert F. Siliciano. Induction
of Human Immunodeficiency Virus Type 1 (HIV-1)-Specific Cytolytic T
Lymphocyte Responses in Seronegative Adults by a Nonreplicating,
Host-Range-Restricted Canarypox Vector (ALVAC) Carrying the HIV-1MN
env Gene, J. Infect. Dis., 171:1623-1627 (1995).

4. Robert. C. Bollinger, Michael. A. Egan, Tae-Wook Chun, Bonnie
Mathieson, and Robert F. Siliciano. Cellular Immune Responses to HIV-1
in Progressive and Non-Progressive Infections. AIDS, 10:1-12 (1996).

5. Michael A. Egan, Lucy M. Carruth, Jennifer F. Rowell, Xiaofang Yu,
and Robert F. Siliciano. HIV-1 Envelope Protein Endocytosis Mediated
by a Highly Conserved Intrinsic Internalization Signal in the
Cytoplasmic Domain of gp41 Is Suppressed in the Presence of the
Pr55gag Precursor Protein, J. Virol., 70:6547-6556 (1996).

Abstracts

1. Michael A. Egan and Robert W. Zoellner.  The Mono-Boro-Analogs of
Acetylcholine: Structural and Electronic Comparisons to the Parent
Acetylcholine Using the MNDO Computational Method.  Annual American
Chemical Society Meeting, San Francisco, CA, April 1992.

2. Michael A. Egan, Kent Weinhold, Enzo Paoletti, James Tartaglia,
Mary Lou Clements, Wendy Pavlat, and Robert F. Siliciano.  Cytotoxic T
Lymphocyte Responses to Recombinant Canarypox-gp160 MN (ALVAC vCP125,
HIV-1 gp160 MN) in HIV-1 Uninfected Adult Volunteers. The 6th Annual
Conference on Advances in AIDS Vaccine Development, Alexandria, VA,
October 30th - November 4th, 1993.

3. Michael A. Egan, Wendy Pavlat, James Tartaglia, Enzo Paoletti,
Robert F. Siliciano and Mary Lou Clements.  Induction of
HIV-1-specific Cytotoxic T Lymphocyte Responses in Seronegative Adults
by a Non-Replicating, Host Range Restricted Recombinant Canarypox
Vector Carrying the HIV-1 env Gene.  The 7th Annual Conference on
Advances in AIDS Vaccine Development, Reston VA, November 6th-10th,
1994.

4. Michael A. Egan, Wedy Pavlat, James Tartaglia, Enzo Paoletti,  Mary
Lou Clements and Robert F. Siliciano.  Cytotoxic T Lymphocyte
Responses to Recombinant Canarypox-gp160 MN (ALVAC vCP125, HIV-1 gp160
MN) in HIV-1 Uninfected Adult Volunteers.  The Frank B. Polk Symposium
on AIDS, Baltimore, MD, April 15th-16th, 1994.

5. Michael A. Egan, Jennifer F. Rowell, Robert F. Siliciano and Lucy
M. Carruth. The Role of the gp41 (YXXL) Internalization Motif in HIV-1
Assembly.", Third Conference on Retroviruses and Opportunistic
Infections, Washington, DC, January 28th - February 2nd, 1996. 

REFERENCES

                        Robert F. Siliciano, M.D., Ph.D.
                        Assistant Professor, Department of Immunology
                        Johns Hopkins University School of Medicine
                        Rm. 1049, Ross Research Bldg. 720 N. Rutland
                        Ave. Baltimore, MD  21205 Office: 410-955-2958
                        Fax:    410-955-0964 e-mail:
                        rsilicia at welchlink.welch.jhu.edu

                        Robert C. Bollinger, M.D., M.P.H.
                        Assistant Professor, Division of Infectious
                        Disease Johns Hopkins University School of
                        Medicine Rm. 1159, Ross Research Bldg. 720 N.
                        Rutland Ave. Baltimore, MD  21205 Office:
                        410-614-0936 Fax:    410-955-7889 e-mail:
                        rcb at welchlink.welch.jhu.edu

                        Xiao-Fang Yu, M.D., Ph.D.
                        Department of Molecular Biology and Immunology
                        Johns Hopkins University School of Hygiene and
                        Public Health 615 N. Wolfe, St. Baltimore, MD
                        21205 Phone:  410-955-3768 Fax:   
                        410-955-0105
----------------------------------------------------------------------
------

-- 
Michael A. Egan
Department of Molecular Biology and Genetics
Ross Research Bldg., Rm. 1050
Johns Hopkins University
720 N. Rutland Ave.
Baltimore MD 21205
Phone: 410-955-7757
Fax: 410-955-0964
email: egan at welchlink.welch.jhu.edu
http://www.welch.jhu.edu/~egan



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