Retrovirus Svedberg value

T. Bauer tbauer at saul9.u.washington.edu
Sun Sep 14 22:57:44 EST 1997


Hi,

I am trying to centrifuge a retrovirus through a tissue culture medium.
According to a Beckman manual, the Sedimentation Coefficient for
a Feline leukemia virus is about 1000 Svedberg units.  I assume this
is probably about the same for the moloney murine leukemia viruses.
Also, the sed. coefficient listed may be for water at 20 deg. C.
Would this value change significantly with tissue culture medium plus
10% serum?

Any assistance would be welcome.
Thanks,

Tom Bauer, Ph.D.
University of Washington

--
tbauer at u.washington.edu




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