Need advice on resolving bacteriophage mix
bmb8msl at leeds.ac.uk
Mon Mar 8 17:15:22 EST 1999
Try using DNase1.
John Scott Meschke wrote in message <7bujr0$49n at net.bio.net>...
>I am currently having a problem resolving a mixture of male-specific RNA
>coliphages from male-specific DNA coliphages. I am interested in
>purifying the RNA phage. I have tried to plaque purify (3 times in
>sequence), as well as plaque purifying using a composite antisera
>against prototype F+ DNA coliphages (again three times in sequence) with
>no luck. It seems to me that these techniques should have worked ( I
>would have bet that I would have better odds of winning the lottery than
>failing with both techniques). Please give me any insight you may have
>regarding the persistence of this mix or anyway to resolve it. Thank
>you very much in advance.
More information about the Virology