Need advice on resolving bacteriophage mix

Mark Landis bmb8msl at leeds.ac.uk
Mon Mar 8 17:15:22 EST 1999


Hey there,

Try using DNase1.

MJSL

John Scott Meschke wrote in message <7bujr0$49n at net.bio.net>...
>I am currently having a problem resolving a mixture of male-specific RNA
>coliphages from male-specific DNA coliphages.  I am interested in
>purifying the RNA phage.  I have tried to plaque purify (3 times in
>sequence), as well as plaque purifying using a composite antisera
>against prototype F+ DNA coliphages (again three times in sequence) with
>no luck. It seems to me that these techniques should have worked ( I
>would have bet that I would have better odds of winning the lottery than
>failing with both techniques).  Please give me any insight you may have
>regarding the persistence of this mix or anyway to resolve it.  Thank
>you very much in advance.
>
>Scott Meschke
>
>






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