Kunjin/Westnile like virus differentiation

jason roberts pteropid1 at ozemail.com.au
Sat Mar 3 06:49:43 EST 2001


Hi everyone,

Just another inquiry into diagnostic testing,

Given the remarkably close relationship between the Kunjin virus and
Westnile virus genetic structure, I was wondering if anyone knows of a
simple method for differentiating between these two viruses (without
resorting to sequencing).

We currently use a PCR detection method, based on universal primers directed
at the NS5 gene (Chang et. al. C.D.C ). I feel that it would be of benefit
to be able to distinguish between the two viruses...

....perhaps someone has come up with a probe for each type??

>From direct observation of the genetic data, it seems like an accurate
differentiation is going to be rather elusive, without resorting to more
complex methodology.

Many thanks.

Jason A. Roberts
Virology Division,
Department of Microbiology.
Prince of Wales Hospital.
Sydney.
Australia.
Robertsjas at sesahs.nsw.gov.au








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