Kunjin/Westnile like virus differentiation

Geoff Crawford access_academix at optusnet.com.au
Fri Mar 9 04:38:08 EST 2001


RE: KUN and WNV

What about glycosylation of proteins from infected cell cultures -
absolutely nothing to do with DNA!  Use tritiated mannose and separate on
SDS-PAGE.  KUN has a set of glycoproteins - gp66, gp 59 & gp 53.  Some
strains of WNV are not glycosylated (?? from memory) and the MW will be
different for those that are!


>From a flavivirus protein dinosaur!!

Dr Geoff Crawford
Access Academix
89 Dellfield Drive
Templestowe VIC 3106
Australia

Phone/Fax:      +61 3 981 272 80
Mobile              +61 412 599 649
Email        access_academix at optusnet.com.au


jason roberts <pteropid1 at ozemail.com.au> wrote in message
news:GZKn6.960$o4.48244 at ozemail.com.au...
> Hi everyone,
>
> Just another inquiry into diagnostic testing,
>
> Given the remarkably close relationship between the Kunjin virus and
> Westnile virus genetic structure, I was wondering if anyone knows of a
> simple method for differentiating between these two viruses (without
> resorting to sequencing).
>
> We currently use a PCR detection method, based on universal primers
directed
> at the NS5 gene (Chang et. al. C.D.C ). I feel that it would be of benefit
> to be able to distinguish between the two viruses...
>
> ....perhaps someone has come up with a probe for each type??
>
> >From direct observation of the genetic data, it seems like an accurate
> differentiation is going to be rather elusive, without resorting to more
> complex methodology.
>
> Many thanks.
>
> Jason A. Roberts
> Virology Division,
> Department of Microbiology.
> Prince of Wales Hospital.
> Sydney.
> Australia.
> Robertsjas at sesahs.nsw.gov.au
>
>
>








More information about the Virology mailing list