plaque assay protocoll ?

gopinath rajuseetharaman drgopin at hotmail.com
Thu May 3 18:03:46 EST 2001


Hi,

I use seaplaque agarose from FMC for overlay for BHV-1 plaque assay.
You can microwave the agarose(which is sufficient) and mix with 2x DMEM or 
similar medium with anitbiotic. I have adapted the protocol from :
http://www.invitrogen.com/Content/Tech-Online/molecular_biology/manuals_pps/maxbacte_man.pdf 
  (Page22-)

You can also try this web site:
http://www.virapur.com/new2431.html

And there is  a software also available for the plaque assay @

http://www.androclus.vet.uu.nl/spotter/spotter.html

Bye,
Gopinath
===========
    Gopinath Raju Seetharaman
    Post-Doctoral Research Fellow,
    University of Nebraska-Lincoln,
    Dept. of Veterinary and Biomedical Sciences,
    East Campus Loop and Fair street,
    Lincoln,NE 68583.
    Tel:402-472-3320

>From: "Frank M. Mattes" <mattes at rfhsm.ac.uk>
>To: bionet-virology at net.bio.net
>Subject: plaque assay protocoll ?
>Date: Thu,  3 May 2001 14:19:35 +0100 (BST)
>
>Dear newsgroup reader,
>
>does anybody know a good protocoll to do a plaque assay for cmv ?.
>I know you need methylcellulose, wich is critical to avoid the spread of 
>the
>virus within the media.
>I'm wondering also if these clellulose media is also pre-mixed available
>from a commerical source.
>
>Many thanks for your help
>
>Frank Mattes
>Dept. of Virology
>RFH
>London
>
>

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