On 18 Mar 2002 09:49:32 -0000,
D. Levy (levy at removespam.uab.edu) wrote:
> I believe that HEK293T (or just 293T as commonly cited) is a subclone
> of HEK293 that is particularly transfectable, hence the "T"
> designation.
No. 293T is a derivative of 293 cells that stably express
the large T-antigen of SV40. Plasmids containing the SV40
origin are replicated to a copy number of between 400 -
1000 plasmids per cell within 293T, and therefore express
at a higher level.
293T are also resistant to G418. This cell-line was made
in Michelle Calos' lab at Stanford about 14 years ago.
Ashok
--
Ashok Aiyar, Ph.D.
Assistant Professor email: a-aiyar at northwestern.edu
Department of Microbiology-Immunology office: (312) 503-2524
303 E. Chicago Avenue, WARD 4-123 lab: (312) 503-2542
Northwestern University, Chicago, IL 60611 fax: (312) 503-1339
