Hek 293T

Ashok Aiyar aiyar at ebv.mimnet.northwestern.edu
Tue Mar 19 04:32:18 EST 2002

On 18 Mar 2002 09:49:32 -0000,
    D. Levy (levy at removespam.uab.edu) wrote:
> I believe that HEK293T (or just 293T as commonly cited) is a subclone
> of HEK293 that is particularly transfectable, hence the "T"
> designation.

No.  293T is a derivative of 293 cells that stably express
the large T-antigen of SV40.  Plasmids containing the SV40
origin are replicated to a copy number of between 400 -
1000 plasmids per cell within 293T, and therefore express
at a higher level.

293T are also resistant to G418.  This cell-line was made
in Michelle Calos' lab at Stanford about 14 years ago.

Ashok Aiyar, Ph.D.
Assistant Professor                    email: a-aiyar at northwestern.edu
Department of Microbiology-Immunology           office: (312) 503-2524
303 E. Chicago Avenue, WARD 4-123                  lab: (312) 503-2542
Northwestern University, Chicago, IL 60611         fax: (312) 503-1339

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