Virus purification / isolation

A Giessauf ldltryptophan at hotmail.com
Fri Dec 5 04:29:21 EST 2003


    Dear all,

    I am about starting to write a proposal for a research project

     about a novel method for isolation / purification of virus particles

     from cell culture supernatants for use as antigens for diagnostic

     assay production.

     I have published the first article on this method "A technique for

     isolation of rubella virus-like particles by sucrose gradient

     ultracentrifugation using Coomassie brilliant blue G crystals" last

      year (Anal.Biochem. 308 (2002) 232-238) and I am searching now

      for

      (1) interested international research partners, who can provide me

      with stable transfected cell lines (or other expression systems)

      that secretes any virus-like particles that may become by my

      research more interesting for use as an antigen for assay

      production. I will try to carry forward the technique from the

      already described method for Rubella virus purification also to

      other virus particles.

      Density gradient ultracentrifugation has to be the final

      purification step.

      (2) Any industrial research partners that are interested to test

       antigens for assay production (e.g. immunoblot test stripes) are

       also welcome and will be mentioned in my research proposal.

       The advantage of an industrial partner in such a collaboration

       will be to obtain antigens at a lower price and the greatest part

       of the developing work will be done by my research work.


			Please contact my as soon as possible if you are

				interested ,

                           any comments or ideas are welcome.


			Sincerely yours,

                                    Andreas Gießauf, PhD
                                    andreas.giessauf at uni-graz.at



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