Virus purification / isolation
ldltryptophan at hotmail.com
Fri Dec 5 04:29:21 EST 2003
I am about starting to write a proposal for a research project
about a novel method for isolation / purification of virus particles
from cell culture supernatants for use as antigens for diagnostic
I have published the first article on this method "A technique for
isolation of rubella virus-like particles by sucrose gradient
ultracentrifugation using Coomassie brilliant blue G crystals" last
year (Anal.Biochem. 308 (2002) 232-238) and I am searching now
(1) interested international research partners, who can provide me
with stable transfected cell lines (or other expression systems)
that secretes any virus-like particles that may become by my
research more interesting for use as an antigen for assay
production. I will try to carry forward the technique from the
already described method for Rubella virus purification also to
other virus particles.
Density gradient ultracentrifugation has to be the final
(2) Any industrial research partners that are interested to test
antigens for assay production (e.g. immunoblot test stripes) are
also welcome and will be mentioned in my research proposal.
The advantage of an industrial partner in such a collaboration
will be to obtain antigens at a lower price and the greatest part
of the developing work will be done by my research work.
Please contact my as soon as possible if you are
any comments or ideas are welcome.
Andreas Gießauf, PhD
andreas.giessauf at uni-graz.at
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