From mgillum from americaschoice.org Wed Mar 5 11:50:39 2008 From: mgillum from americaschoice.org (Mardi Gillum) Date: Thu Mar 6 13:27:08 2008 Subject: [Virology] FIBROPAPILLOMAS WEBSITE Message-ID: Hello, Do you know how I would contact Ursula Keuper-Bennett besides using the email addresses on turtle.org site. We would like to obtain print quality photos of "Howzit" to use with her article for a student reading in a literacy lesson. We can not seem to get a reply from the turtle.org emails. Thank you, Mardi Gillum America's Choice, Inc. www.americaschoice.org From ukats02 from yahoo.com Mon Mar 17 16:21:36 2008 From: ukats02 from yahoo.com (ukats) Date: Tue Mar 18 13:25:58 2008 Subject: [Virology] Determination of particle/pfu ratio Message-ID: <776807.59532.qm@web51303.mail.re2.yahoo.com> Dear all, I have OD 260 value and PFU/ml value of purified virus sample. How then do I calculate particle/pfu ratio using these values? Thanks, Ukats. From bbx107 from excite.XXXX.com Sat Mar 22 11:18:09 2008 From: bbx107 from excite.XXXX.com (bob) Date: Mon Mar 24 12:38:35 2008 Subject: [Virology] Re: Determination of particle/pfu ratio References: Message-ID: <27cau3tnpdmucb3j5et4o9jui0a3v5d9u4@4ax.com> On Mon, 17 Mar 2008 14:21:36 -0700 (PDT), ukats wrote: >Dear all, > >I have OD 260 value and PFU/ml value of purified virus >sample. How then do I calculate particle/pfu ratio >using these values? > So, do you know how many virus particles corresponds to 1 OD unit? If not, you will need to measure it. Electron microscopy? Or some labeling. bob From randhawapa from upmc.edu Mon Mar 24 13:10:39 2008 From: randhawapa from upmc.edu (Randhawa, Parmjeet) Date: Wed Mar 26 12:00:46 2008 Subject: [Virology] Re: Determination of particle/pfu ratio In-Reply-To: <27cau3tnpdmucb3j5et4o9jui0a3v5d9u4@4ax.com> References: <27cau3tnpdmucb3j5et4o9jui0a3v5d9u4@4ax.com> Message-ID: <58085D8E8D09D34497D5595559B1A38B0E1794BF@1upmc-msx5.acct.upmchs.net> If you know the size in base pairs of the virus, you can calculate the molecular weight of the viral DNA (1 nucleotide PAIR= 660 daltons). Viral DNA concentration (calculated from OD 260 value) can then be converted to viral particles using Avogadro's number (1 Gram MW= 6.02e+23 viral particles). Parmjeet Randhawa, M.D. Professor of Pathology, Division of Transplant Pathology, University of Pittsburgh, Department of Pathology, E737 UPMC-Montefiore Hospital, 3459 Fifth Ave, Pittsburgh, PA 15213. Phone: 412 647 7646 Fax: 412 647 5237 -----Original Message----- From: virology-bounces@oat.bio.indiana.edu [mailto:virology-bounces@oat.bio.indiana.edu] On Behalf Of bob Sent: Saturday, March 22, 2008 12:18 PM To: bionet-virology@magpie.bio.indiana.edu Subject: [Virology] Re: Determination of particle/pfu ratio On Mon, 17 Mar 2008 14:21:36 -0700 (PDT), ukats wrote: >Dear all, > >I have OD 260 value and PFU/ml value of purified virus >sample. How then do I calculate particle/pfu ratio >using these values? > So, do you know how many virus particles corresponds to 1 OD unit? If not, you will need to measure it. Electron microscopy? Or some labeling. bob _______________________________________________ Virology mailing list Virology@net.bio.net http://www.bio.net/biomail/listinfo/virology From nashwa_said_2008_200100 from yahoo.com Mon Mar 24 16:32:54 2008 From: nashwa_said_2008_200100 from yahoo.com (nashwa said) Date: Wed Mar 26 12:00:53 2008 Subject: [Virology] enquiry Message-ID: <662030.26865.qm@web57208.mail.re3.yahoo.com> Dear sir We are acompany located in Kuwait ,we need to ask about Avian ILT infectious lyrangiotrachitis virus - FITC conjugated antiserum for the ministery of agriculture if it is available please replay me as soon as possible Best regardes Dr Nashwa Said __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From crispinthiley from yahoo.co.uk Tue Mar 25 07:10:24 2008 From: crispinthiley from yahoo.co.uk (crispinthiley@yahoo.co.uk) Date: Wed Mar 26 12:00:58 2008 Subject: [Virology] TCID50 Message-ID: <5774e4b6-065b-40f7-bb2f-f9b35fe29a0a@e10g2000prf.googlegroups.com> Hi, Any recommendations. I am doing so viral replication assays under hypoxia and normoxia. This is causing my cells to grow a different rates through the time points. I am trying to nomalise the data. Should i normalise to cells at time of infection as standard. At 24 hours, for cell count at each time point or use a ration of cell growth under different conditions. I have counted cell number under different conditions at all time points. Stuggling to find any detail in the literature to support this. Many Thanks Crispin From DrKJRoberts from gmail.com Mon Mar 31 13:09:52 2008 From: DrKJRoberts from gmail.com (DrKJRoberts@gmail.com) Date: Mon Mar 31 13:19:27 2008 Subject: [Virology] International Science and Engineering Fair - Call for Judges Message-ID: <20414554-3081-4b89-9709-aa7e1f0dcee6@13g2000hsb.googlegroups.com> The 59th ISEF will be held this year in Atlanta, Georgia May 11th-17th. I am looking for volunteers to serve as Grand Awards Judges in the Microbiology Category. Last year's ISEF in Albuquerque, New Mexico, over 45 different countries sent 1510 secondary school finalists to compete. We expect the same turn out at this year's Fair. I am looking for judges to choose the top finalists who will compete for thousands of dollars in prizes and awards. Grand Awards Judges will arrive in Atlanta on Tuesday, May 13 for orientation and pre-judging and will judge on Wednesday, May 14. This is the premier worldwide science research competition for high school students grades 9-12 and we need your support. If you are interested in serving as a judge at this year's ISEF, please go to the following website and register: http://www.intelisef2008.org/judging.html If you need more information, don't hesitate to contact me at drkjroberts@gmail.com Karl J. Roberts, Ph.D. Co-Chair, Microbiology Division International Science and Engineering Fair