Dana Ann Dudle
ddudle at indiana.edu
Fri Jun 27 09:58:17 EST 1997
To avoid problems with ethidium bromide, I always cut the gel before I
stain... this works if you are doing population-level studies where you
know where the bands you are interested in will be on the gel. THis
saves MUCH money without being dangerous. Another thing we did (in a
well funded but frugal lab) was save TBE and TAE for a week, in beakers
near the gel rigs. I don't know if this is standard procedure or not,
but it definitely seemed to work. This actually probably saves more time
than money, however. Still valuable, though!
By the way-- I never imagined NOT racking my own pipet tips. Maybe I
don't deal in the volume that some of you do, however.
Debra M. McDonough (donough at UCSUB.COLORADO.EDU) wrote:
> Repour agarose gels. Unless you need to blot the gel or cut bands from
> it, you can usually remelt, repour and reuse a gel several times. It's
> actually easier than weighing more agarose!
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