Question: Blood (serum) clots - Help!

Kimberly Walker walker at umbi.umd.edu
Tue Apr 21 23:06:13 EST 1998


When I was a med tech, and we had those problems, we allowed the natural 
clot to form longer.  So, RT for, say, 2 hours, then overnight in the 
refrigerator.  
Could you use EDTA or something?

Good luck

Kim


On 14 Apr 1998, Laurie Davison wrote:

> Hi:)
>    I'm posting this question to a few newsgroups in hopes that someone 
> can answer a question for me.
>    I work in an equine reproductive research laboratory and we do a lot 
> of blood work - mainly RIA of various hormones in mare's blood. We treat 
> all blood samples the same way: allowed to clot at room temp for about an 
> hour, then placed in the 'fridge overnight to allow the clot to shrink, 
> then centrifuged and poured off the following morning. Heparin cannot be 
> used as it interferes with two of our assays (melatonin and T4).
>    The problem is that we keep getting large "serum clots" (presumably 
> due to fibrin?) which are causing us to lose up to 80% of the total 
> volume of serum. Their formation does not seem to be affected by the 
> ammount of time we keep it at room temp or in the 'fridge, or by the 
> speed of centrifugation. Since we take our blood at a University farm and 
> have to travel 20 minutes back to the lab, "immediate" centrifugation for 
> plasma is also not an option. 
>    Have any of you run into this problem in your work? If so, can you 
> offer any advice on how to prevent this from occurring? I've worked with 
> the blood of a number of species and I don't recall ever having had this 
> trouble before.
>    Suggestions *Gratefully* Awaited!!!:)
> 
> -Laurie-
> 
> 
> 




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