Postdoc-pollen receptor kinases

Sheila McCormick sheilamc at nature.berkeley.edu
Wed Sep 19 04:22:46 EST 2001


Postdoc Position - Signaling via pollen receptor kinases, Plant Gene 
Expression Center, UC-Berkeley, Albany, CA
We are studying the signaling pathways mediated by pollen-specific 
receptor-like kinases in tomato (Plant Cell 10:319-330, 1998). In recent 
work, we have used the yeast two-hybrid system to identify ligands and 
downstream protein partners for these kinases. We have confirmed one of the 
ligand-kinase interactions using co-immunoprecipitation assays (see 
Abstract from ASPP mini-symposium at
http://www.rycomusa.com/aspp2001/public/M14/0953.html), and we have 
confirmed the interaction between the kinases and one downstream component, 
a potentially highly phosphorylated but otherwise uncharacterized protein, 
KPP1 (kinase partner protein). A 2nd round Y2H screen with KPP revealed 
potential interactions with cytoplasmic components already implicated in 
pollen tube growth. The postdoctoral fellow will carry out experiments to 
decipher the downstream components of the signaling pathway(s), including 
pulldown assays, deletion analyses and phenotypic and biochemical analyses 
of T-DNA insertions in the Arabidopsis homologs of the kinase partner 
proteins. Training and a publication record in biochemistry and molecular 
biology is required.  Prior experience with reproductive biology and/or 
kinases is a plus, but prior experience with plants is not necessary. 
Familiarity with bioinformatics is desirable. Funding is available for at 
least 2 years, but the potential for obtaining partial salary support via 
fellowship applications will be considered in applicant selection. Position 
available now, although a later start date can be negotiated; open until 
filled. Salary ~$30K. For consideration send a CV with a list of 
publications, a short statement of research interests and the names and
e-mail addresses and/or phone numbers of 3 references to: 
sheilamc at nature.berkeley.edu or to Sheila McCormick, Plant Gene Expression 
Center, 800 Buchanan St., Albany, CA 94710; FAX (510)-559-5678
For more information about the other projects in the lab, see 
http://www.pgec.usda.gov/McCormick/mclab.html


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