structural refinement using low temperature data

ong at BCL7.BIOCHEM.UGA.EDU ong at BCL7.BIOCHEM.UGA.EDU
Wed Apr 10 18:35:45 EST 1996


Hi,
	Does anyone has experience of structural refinement using low
temperature data? I try to refine my protein structure with low temperature
data, but the r value is about 0.3, which can't go down further without B-value
refinement.
	maybe you could give me a good recommend about the strategy of
refinement. I am still curious to the reason why the better data works worse.
Does the error come from data processing?
	Recently, A lot of x-ray machinces were installed with low-temperature
device. I though how to deal with low temperature data is very important and
serious topics.

	Ping-lin Ong
	Department of Biochemistry and Molecullar biology
        University of Georgia
        Athens, Georgia 30602
 	U.S.A.

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