Making a map of protein-DNA complex

Chad C. Sines chad.sines at chemistry.gatech.edu
Sun Aug 13 16:48:20 EST 2000


I am mainly a nucleic acid crystallographer and have a very limited experience with proteins and CNS.  I am wanting to make a map of a protein-DNA complex that is in the PDB to look at the DNA-protein interface.  I have downloaded the structure factors and generated the mtf file. The problem is that ~133 of the 8000 atoms are unobserved/disordered and not in the pdb file.  Generate_easy is placing them in the mtf and pdb file based on their bond and angle restraints.  How can I avoid this so that I can make a usable maps?  

Chad






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