Crystallography of nonheme metalloenzymes

Douglas Ohlendorf ohlen at dcdiff.med.umn.edu
Mon Jul 31 15:42:54 EST 2000


POSTDOCTORAL POSITION TO STUDY NONHEME METALLOENZYMES
DIETRICH KAHLERT STRUCTURAL BIOLOGY LABORATORY
UNIVERSITY OF MINNESOTA, MINNEAPOLIS MN 55455

A postdoctoral position is immediately available at the Kahlert Structural
Biology Laboratory of the University of Minnesota to study the relation of
function and structure of metalloenzymes that use a nonheme iron cofactor.
The current major emphasis of this work is the catechol dioxygenases. Over
50 mutants of these proteins have been generated and are being purified and
studied. Thus far 19 separate mutants have been crystallized and their
structures in the presence of 3-4 substrates/inhibitors have been made. The
successful candidate will be responsible for continuing this work primarily
through purification, crystallization, and structural analysis. This work is
in collaboration other members of the Center for Metals in Biocatalysis
(http://bioinorg.chem.umn.edu/) which is composed of approximately 70
researchers in 8 laboratories at the University of Minnesota. The Center for
Metals in Biocatalysis is among the leading centers for the study of
metalloproteins.

The available crystallographic facilities include an R-AXIS IV imaging plate
system with MaxFlux Blue confocal optics, 2 Siemens multiwire systems, 2
Rigaku rotating anodes with 2 liquid N2 crystal coolers and a robotic
crystallization system. A crystal microspectrophotometer will be installed
this fall. Synchrotron beamtime is available at ALS through the university's
membership in the Molecular Biology Consortium
(http://elindy.el.anl.gov/mbc/Home/index.html). Superb computational
resources are available through the Minnesota Supercomputing Institute
(http://www.msi.umn.edu/) which maintains the Basic Sciences Computing
Laboratory (http://www.msi.umn.edu/bscl/hardware/) adjacent to the Kahlert
Structural Biology Laboratory.

The position is funded through 2003 with a salary commensurate with
experience. Applicant must have demonstrated experience in macromolecular
crystallography as well as in protein purification. Other projects ongoing
in the lab include the structural analysis of proteins involved in the
virulence of gram-positive pathogens.

For additional information or to make application contact:

Douglas H. Ohlendorf                                       Phone:
612-624-8436
Professor                                                  FAX:
612-624-5121
Dept. of Biochemistry, Molecular Biology & Biophysics
Twin Cities Campus, University of Minnesota
EMAIL:
ohlen at dcdiff.med.umn.edu
Lab web site:
http://biosci.cbs.umn.edu/bmbb/ohlen_lab/index.html


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