Workshop: Crystallization: focus on membrane proteins; April 15 d ead line approaching

Stojanoff, Vivian stojanof at bnl.gov
Mon Apr 12 09:17:42 EST 2004


              Crystallization: focus on membrane proteins

                   http://protein.nsls.bnl.gov
                               
        http://www.nsls.bnl.gov/users/meeting/2004/workshop8.htm

The workshop will cover different crystallization methods from the most
standard vapor diffusion methods to more advanced techniques. A short
introduction to the crystallization process will be followed by a series of
hands-on experiments. Participants will be able to crystallize there own PSI
and PSII proteins and use the lipid cubic phase to crystallize their own
samples. The goal is for all participants to be able to try out the
different methods under discussion. Two topics will be covered:  
 
 
 - Soluble Proteins  
 Myrka Dauter Vapor diffusion - heavy atom soaks  
 Marie Claude Marchand Vapor diffusion techniques - seeding techniques  
 Naomi Chayen Microbatch and Containerless oil techniques  
 Ana Merlo  Counter diffusion Mehtods.   
 Zbigniew Dauter Heavy atom soaking and strategies.   
 
 
 - Membrane Proteins 
 Petra Fromme PSI and PSII   
 Peter Nollert Lipid Cubic Phase  
 Naomi Chayen Crystallisation in oil  
 
 
The workshop is limited to 20 participants due to its hand on character. It
is on a first come first serve basis. Participants are welcome to bring
their own samples.

All participants of this workshop need a VALID BNL GUEST NUMBER since the
workshop will take place on the experimental floor. Please make sure to
register for both: the workshop and to obtain a valid BNL Guest appointment.
Due to increased site security foreing nationals need to allow at least 30
days for security clearance.
(http://www.nsls.bnl.gov/users/meeting/2004/register.asp
 https://fsd84.bis.bnl.gov/guest/)
 
 
 
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