Many thanks to all of those who sent me the co-ordinates of 1MAD. They will
be very useful to one of my co-workers as will the information about the EMBL
fileserver (reproduced below as I thought others may find it useful too!).
___________________________BEGIN INCLUDED MESSAGE____________________________
Dear Craig,
in order to get up-to-date atomic coordinates from the Protein Data Bank
in Brookhaven the easiest thing for you to do would probably be to have
them sent from the EMBL fileserver in Heidelberg (e-mail address :
FILESERV at EMBL-Heidelberg.de). In case you have problems to get the
files across to JANET let me know and I'll mail them to you just like
this message (I'd be very surprised if you had difficulties though).
I have enclosed the reply to a message with the lines HELP and
HELP PROTEINDATA to give you an idea of what's available.
In case you have access to Internet you would also have the possibility
to download pre-release entries. The address is pdb.pdb.bnl.gov
(130.199.144.1).
Regards, Hans.
Original messages follow :
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HELP [GENERAL]
DIR [GENERAL]
The EMBL Network File Server enables network access to the following:
- EMBL Nucleotide Sequence Database.
- The *newest* nucleotide sequence data created at EMBL *and* GenBank
since the latest full release of the EMBL database.
- DNA sequence alignments and consensus sequences
- Swiss-Prot Protein Sequence Database
- Brookhaven Protein Database and other structure data
- Prosite Pattern Database
- Transcription Factor Database (TFD)
- Eukaryotic Promotor Database (EPD)
- tRNA sequence and tRNA gene sequence database
- E. coli Database (ECD)
- LiMB (Listing of molecular biological databases) Database
- Restriction Enzyme Database (REBASE)
- ENZYME Database
- Drosophila Genetic Map Database (FlyBase)
- Alu sequence database
- Codon usage tables
- Free software for molecular biologists
- Reference lists of relevance to molecular biology
- General documents with importance to molecular biology
- Documents describing the services of the EMBL Data Library
Using the File Server
- ---------------------
You use the file server by sending one or more of the commands shown below
as electronic mail to the address NETSERV at EMBL-Heidelberg.DE. The requested
file(s) will then be returned to you by the file server. You must find out
which operating system command you should use on your own computer in order
to send electronic mail and how to format the address
NETSERV at EMBL-Heidelberg.DE The mail which you send to the file server
should contain one file server command per line.
Commands
- --------
The Network File Server at the European Molecular Biology Laboratory supports
the following commands:
Help - sends a general help file (this file)
Help <topic> - sends the help file and directory
listing of <topic>
Get <filename> - sends the file <filename>
Dir <directory> - same as Help <directory>
Directories
- -----------
The files accessible via the file server are organised into directories.
The following directories exist:
Nuc - Nucleotide Sequence data from
the EMBL and GenBank sequence databases.
Prot - The SWISS-Prot Protein Sequence Database
ProteinData - Protein structure data
ProSite - ProSite Pattern Database
ECD - E. coli Database
ENZYME - The ENZYME database
TFD - Transcription Factor Database
EPD - Eukaryotic Promotor Database
LiMB - Listing of Molecular Biological Databases
REBASE - Restriction Enzyme database
tRNA - tRNA database
Flybase - Drosophila Genetic Map Database
Alu - Alu sequence database
CodonUsage - Codon usage tables
Align - DNA sequence alignments and consensus sequences
RefList - Bibliographies, Reference lists
DOS_Software - Free software for IBM PCs and clones
Mac_Software - Free software for the Apple Macintosh
VAX_Software - Free software for VAX/VMS
UNIX_Software - Free software for UNIX
Software - General software and software information
Doc - Documents with relevance to molecular biology
(Submission forms, technical documents etc.)
In general, the <filename> in the GET command begins with the name of
the appropriate directory, followed by a colon. There should be no embedded
blanks (spaces) between the directory name, the colon and the filename.
Help
- ----
Further help is available on the following topics:
Nuc Prot ProteinData ProSite ECD
ENZYME EPD LIMB Doc REBASE
RefList Software DOS_Software Mac_Software VAX_Software
UNIX_Software Flybase Align CodonUsage TFD
Alu tRNA
Examples
- --------
To request:
- specific help on the sequence databases
- general help on software
- the sequence called PIP03XX (nucleotide)
- the sequence with accession number X03392 (nucleotide)
- the sequence called KAP$YEAST (protein)
- the new nucleotide sequence citation index (NEWCITATION.NDX)
- the sequence submission form
you could create a file containing the file server commands:
HELP NUC
HELP PROT
HELP SOFTWARE
GET NUC:PIP03XX
GET NUC:X03392
GET PROT:WAP$MOUSE
GET NUC:NEWCITATION.NDX
GET DOC:DATASUB.TXT
and then mail that file to the address NETSERV at EMBL-Heidelberg.DE
Alternatively, you could type file server commands directly into
your mail program.
Please note that if the command you use to send mail allows you to specify a
"subject" then anything you enter as the "subject" will be treated as a file
server command.
File splitting
- --------------
Some files available from NETSERV are much larger than 100k. Although this
should be no problem for today's computer network technology, there are
still some mailer systems which refuse to transport "large" files. To
accommodate most of our users we automatically split files in packets of
approx. 95k. The Subject lines of the messages received from NETSERV give
you information about how many packets were sent and about the part number
of each packet. This should allow you to reconstruct the original file by
joining the individual parts.
Please remember that the speed of mail file transfer normally depends on
the file size. Therefore, you will probably receive smaller files much
faster than larger files. This is of course also true for split files where
the last part will travel faster than the rest. If you receive the last
part, but *not* the others, you should check with your local computer centre
whether there are any limitations on the size of files you are allowed to
receive at your site.
Unfortunately, there may be cases where you will not be able to receive files
above a given size because mailers between EMBL and your site refuse to
transfer them.
News
- -----
Documentation of changes to the file server will be reported in the 'EMBL
File Server News', available in the DOC directory of the file server: eg
GET DOC:EMBL_Server_News.1
Feedback
- --------
If you have any problems using the file server, or suggestions as to how we
might improve the service, please mail to the address
NETHELP at EMBL-Heidelberg.DE. We want to hear from you!
Summary of directories available on the EMBL File Server:
- --------------------------------------------------------
Databases:
EMBL Nucleotide Sequence Database NUC
(Rel. 28, Sep 91 + newest data from EMBL/GenBank/DDBJ)
Eukaryotic Promotor Database (Rel. 28, Sep 91) EPD
SwissProt Protein Sequence Database PROT
(Rel. 19, Sep 91 + newest data)
Prosite pattern database (Rel. 7.10, Aug 91) PROSITE
ENZYME database (Rel. 6.00, Aug 91) ENZYME
Brookhaven Protein Structure Database (Rel. 57, Jul 91) PROTEINDATA
REBASE, Restriction Enzyme Database (Rel. 9110.10, Oct 91) REBASE
tRNA sequence and gene sequence database (1991) TRNA
TFD, Transcription Factor Database (Ver 3.0, Jun 91) TFD
The E.coli Database (Rel. 8, Aug 91) ECD
Drosophila Genetic Map Database (9107, 27-Jul-1991) FLYBASE
Listing of Molecular Biology Databases, LiMB (Rel. 2.0) LIMB
Sequence analysis bibliography (SEQANALREF Rel. 21, Oct 91) REFLIST
Functional Analysis of Nucleotide Sequences bibliography REFLIST
(FANS_REF Rel. 3.4, Apr 91)
Alu sequence database and alignment ALU
Software:
Software for MS-DOS computers DOS_SOFTWARE
Software for Apple Macintosh MAC_SOFTWARE
Software for UNIX UNIX_SOFTWARE
Software for VAX/VMS VAX_SOFTWARE
Other software, GenBank Clearinghouse, etc. MISC_SOFTWARE
Miscellaneous:
Technical documents, submission and order forms, etc. DOC
Multiple DNA sequence alignments and consensus sequences ALIGN
Codon Usage tables CODONUSAGE
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21-Oct-1991
