neophyte

[CZJ at CU.NIH.GOV]

>
>
> i want to try crystallizing a membrane protein, but have no clue how to...
>
> Any good books to read?
> Hints?
>
> thanx
>
> -------------------------------------------------------------------------
> Mark Soekarjo                          Living in a postcard
>
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> -------------------------------------------------------------------------
> Mark Soekarjo                          Living in a postcard
>
> postcards to   la Maison Rose          e-mail to       kh at litsun.epfl.ch
>                Ch. des Clos 53         fax             +41'21'693'41'11
>                CH-1024 Ecublens        voice           +41'21'693'37'02
>                ****europe*****
>
>

One of the major problems in structure determination is the
determination of the structures of membrane proteins.  Success
has been achieved where the soluble portion can be cleaved off and
the structure of that portion determined.  The structures
of true membrane proteins are few and far between.  The major reason is
the difficulty in obtaining diffraction quality crystals.  Some success
has been reported with two dimensional crystals for use
in electron diffraction.

Good Luck!!

Jim Cassatt