Add detergents?
I usually add salt and it has worked in a couple of cases.
Change the construct.
Cheers, Mark
> like probably everyone else, from time to time i've had problems with
>proteins "oiling" out in crystallization drops. i've asked several people
>what they like to do in this situation & answers seem to group into 2
>major catagories: 1) increase ionic strength & 2) decrease ionic strength.
>any other ideas about what to do in general? any arguments to support/
>shoot down the two ideas above?
> -dave.
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Dr. Mark A. Saper saper at umich.edu
Biophysics Research Division saper at umichum.bitnet
University of Michigan Phone: (313) 764-3353
2200 Bonisteel Blvd., Ann Arbor, MI 48109-2099 FAX: (313) 764-3323
