proteins "oiling" out (REPLY)

[Mark A. Saper]

Add detergents?  

I usually add salt and it has worked in a couple of cases.  

Change the construct.

Cheers, Mark

>    like probably everyone else, from time to time i've had problems with
>proteins "oiling" out in crystallization drops.  i've asked several people
>what they like to do in this situation & answers seem to group into 2
>major catagories:  1) increase ionic strength & 2) decrease ionic strength.
>any other ideas about what to do in general?  any arguments to support/
>shoot down the two ideas above?
>                                       -dave.

------------------------------------------------------------------------------
Dr. Mark A. Saper                                              saper at umich.edu
Biophysics Research Division                              saper at umichum.bitnet
University of Michigan                                   Phone: (313) 764-3353
2200 Bonisteel Blvd., Ann Arbor, MI 48109-2099             FAX: (313) 764-3323